- Original Article
- Susceptibility of rat hippocampal neurons to hypothermia during development
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Kyung Ah Seo, Sehhyun Kim, Na Mi Lee, Soo Ahn Chae
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Clin Exp Pediatr. 2013;56(10):446-450. Published online October 31, 2013
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Purpose This study evaluated the extent of damage due to hypothermia in the mature and immature brain. MethodsHippocampal tissue cultures at 7 and 14 days in vitro (DIV) were used to represent the immature and mature brain, respectively. The cultures were exposed at 25℃ for 0, 10, 30, and 60 minutes (n=30 in each subgroup). Propidium iodide fluorescent images were captured 24... |
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